Determination of the temperature mode, duration of presence of the human adenovirus on orbital space stations and influence of clinorotation on some properties of the virus
|1Nosach, LM, 1Dyachenko, NS, 1Tarassishin, LO, 1Zhovnovata, VL, 1Butenko, SI, 1Povnitsa, OYu. |
1Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kiev, Ukraine
|Kosm. nauka tehnol. 2003, 9 ;(1):096-101|
|Язык публикации: Russian|
It is found that the human adenovirus of type 2 is rather tolerant to the influence of temperature as it keeps infectivity during 60 days at a temperature from +20 to +22 °С and during 90 days at a temperature of +4 °С. Some fall decrease of antibody-binding activity of subgenus- and genusspecific antigenic determinates of hexon protein of the capsid of the virus is revealed at a temperature from +20 to +22 °С. The activity of both determinantes of the hexon of the adenovirus exhibited at a temperature of +4 °С during 10 and 20 days is kept practically completely. This testifies that the human adenovirus is suitable for space biology experiments aboard spacecrafts and this point should be taken into account at designing the «Virus» device in which the human adenovirus can be exhibited on the orbit at a temperature of +4 °С or from +20 to +22 °С. The temperature depends on space flight conditions but the first temperature mode is preferable for the preservation of the antigenic structure of the hexon. It is shown that the infectivity of the human adenovirus type 2 is kept under the conditions of horizontal clinorotation during 10—90days. At the clinorotation during 10 and 20 days, it is marked some decrease of antibody-binding activity of subgenus antigenic determinant of hexon. This is evidence for the certain stability of adenoviruses to microgravitalion and will allow one to isolate the influence of other factors under real space flight conditions.
|Ключевые слова: clinorotation, human adenovirus, orbital space stations|
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