Influence of clinostating on the curly potato dwarf virus in vitro and in vivo

1Didenko, LF, 1Parkhomenko, NI, 1Maksimenko, LA, 1Dyachenko, NS, 2Zaritskiy, NM, 2Kozar, FE
1Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kiev, Ukraine
2Institute of Agricultural Microbiology and agricultural production NAAS, Chernіgіv, Ukraine
Kosm. nauka tehnol. 1999, 5 ;(5):118–122
Publication Language: Russian
It is shown that the phytorhabdovirus the curly potato dwarf virus (CPDV) retains its infection properties under the clinostating conditions. However, the structural virus proteins synthesized in plants infected before and after clinostating differ by their quantity ratio characteristic for the native virus.
Keywords: clinostating conditions, native virus, structural virus proteins
1. Kozar F. E., Kurbala M. L., Shcherbina N. V., Zaritskii N. M. Curly dwarfism, a  potato viral diseases caused by a bacilloform virus from the rhabdovirus group.In Virusnye bolezni sel'skohozjajstvennyh kul'tur, 69—76 (Moscow, 1980) [in Russian].
2. Maksimenko L. A., Kraev V. G., Didenko L. F., et al. Issledovanie RNK-zavisimoj RNK-polimerazy virusa kurchavoj karlikovosti kartofelja. Microbiologichny Zhurnal, 56 (5), P. 90 (1994) [in Russian].
3. Chang D., Paulsen A., Johnson T. C., et al. Virus protein assembly in microgravity. Adv. Space Res., 13, 251—257 (1993).
4. Didenko L. F., Parkhomenko N. I., Maksimenko L. A., et al. Physico-chemical and functional peculiarity of phytorhabdo-virus curly potato dwarf virus (CPDV). Bioresourses and viruses: II International conference, P. 120 (Kyiv, 1998).
5. Grubman M. J., Ehrenfeld. E., Summers D. F. In vitro synthesis of proteins by membrane-bound polyribosomes from vesicular stomatitis virus infected Hela-cells. J. Virol., 14, 560—571 (1974).
6. Kordyum E. L. Efects of altered gravity on plant cell processes: results of recent space and clinostatic experiments. Adv. Space Res., 14 (8), 77—85 (1994).
7. Laemmli V. K. Cleavage of Structural proteins during the assembly o the head of Bacteriophage T4. Nature, 227 (5259), 620—685 (1970).
8. Milner J. J., Jackson A. O. Sequence complementarity of Sonchus yellow net virus RNA with RNA from polysomes of infected tobacco. Virology, 97 (1), 90—99 (1979).
9. Moore R. How effectively does a clinostat mimic the ultrastruc-tural efects of microgravity on plant cells? Ann. Bot. (USA), 65 (2), 213—216 (1990).
10. Murphy F. A., Fauquet C. M., Bishop H. L., et al. Virus Taxonomy Classification and Nomenclature of Viruses Sixth Report of the International Committee on Taxonomy of Viruses (Virology Division International Union of Microbiologycal Societies). Archives of Virology, Supplement 10, 275—288 (Springer Verlag, Wien, New York, 1995).
11. Ouchterlony O. In handbook immunodiffusion and immuno-electrophoresis. Ann. Arbor. Michigan. Ann. Arbor. Science Publishers, P. 37 (1968).

12. Wagner R. R. Reproduction of rhabdovirus. In comprenensive virology. Eds H. Fraenkel-Conrat, R. R. Wagner, 1—93 (Plenum Press, New York, 1975).