Using synaptosomes from rat cerebellum and cerebral hemispheres, we investigated the effect of hypergravity on the kinetic parameters К_м and V_max of the Na⁺-dependent, high-affinity L- [¹⁴С] -glutamate transport activity. The hypergravity stress was created by centrifugation of rats for 1 hour at 10g. We observed no differences in  К_м values for cerebellum and cerebral hemisphere synaptosomes between the control rats — 18.2±7.6 μM (cerebellum), 10.7± ±2.5 μM (cerebral hemispheres) and the animals exposed to hypergravity — 23.3± 6.9 μM (cerebellum), 6.7±1.5 μM (cerebral hemispheres). The similarity of this parameter for the two groups of animals showed that the affinity of glutamate transporter to substrate in cerebellum and cerebral hemispheres was not sensitive to hypergravity stress. The maximal velocity of  L- [¹⁴С] -glutamate uptake (V_max) diminished for cerebellum synaptosomes from 9.6±3.9 nmol/min/mg of protein in the control group to 7.4±2.0 nmol/min/mg of protein in the animals exposed to hypergravity stress and for cerebral hemisphere synaptosomes from 12.5±3.2 nmol/min/mg of protein to 5.6±0.9 nmol/min/mg of protein, respectively. It was also shown that the hypergravity effect on the level of L- [¹⁴С] -glutamate uptake was different for cerebellum and cerebral hemisphere synaptosomes. Only for cerebellum synaptosomes, a significant decrease in the level of L- [¹⁴С] -glutamate uptake was observed. Possible mechanisms of the attenuation of glutamate transporter activity are discussed.