Plant cell transformation with agrobacterium tumefaciens under clinorotation
|1Sarnatzkaya, VV, 1Hladun, HO, 1Padalko, SF |
1Institute of Plant Physiology and Genetics, NAS of Ukraine, Kyiv, Ukraine
|Kosm. nauka tehnol. 2005, 11 ;(5-6):115-121|
|Publication Language: Ukrainian|
To investigate microgravity (clinorotation) effect on plant cell transformation with Agrobacterium tumefaciens and crown gall tumor formation, the culture of primary explants of potato and Jerusalem artichoke tubers was used. It is found that the efficiency of tumor formation and development in clinorotated explants are considerably reduced. When using the explants isolated from potato tubers clinorotated for 3, 5 and 19 days, drastic reduction of formation and development of crown gall tumors was observed. Conversely, the tumor number and their development were increased when potato tubers were clinorotated for one day. Four-hour clinorotation of explants inhibits chromatin activation (increase in availability of nuclei to acridine orange) and did not induce any appreciable effect on RNA-polymerase I and II. At one-day clinorotation of potato tubers a considerable increase in template accessibility of chromatin and in activity of RNA-polymerase I and II occurred. We discuss the possibility to use short-term clinorotation of plant organs, from which explants for the transformation with A. tumefaciens will be isolated, for the increase in transformation efficiency of recalcitrant plants.
1. Artemenko O. A., Troyan V. M., Azarskova M. V. The influence of clinorotation on chromatin conformation state and first cell cycle kinetics by pea seeds germination. Ukr. bot. zhurn., 62 (1), 122—130 (2005) [in Ukrainian].
2. Rodriguez R. L., Denhardt D. T. Agricultural Biotechnology: Vector Systems for Molecular Cloning, 534 p. (Agropromizdat, Moscow, 1991) [in Russian].
3. Rubin B. A., Ladygina M. E., Voronkov L. A., et al. Physiological state of tumor tissue induced by Agrobacterium tumefaciens II.
In: Biological studies biosatellite Cosmos, 126—130 (Nauka, Moscow, 1979) [in Russian].
4. Sarnatskaya V. V. Physiological aspects of tumor growth of plants, 150 p. (Nauk. dumka, Kyiv, 1993) [in Russian].
5. Sytnik K. M., Kordyum E. L., Nedukha E. M., et al. The plant cell under the changing geophysical factors, 135 p. (Nauk. dumka, Kiev, 1984) [in Russian].
6. Halstead T. W., Dutcher F. R. Plants in space. Ann. Rev. Plant Physiol., 38, 317—345 (1987).
7. Klaus D. M. Gravitational influence on biomolecular engineering processes. In: Proceedings of 19th ASGSB Annual Meeting, Huntsville, Alabama, November 12—16, 2003, No. 48 (2003).
8. Kordyum E. L. Plant growth and development in microgravity. In: Proc. of the International Conf. on Plant Onthogenesis in Natural and Transformed Environments, Lviv, July 1—4, 1998, 11 — 13 (1998).
9. Kordyum E. L., Sytnik K. M. Biological effects of weightlessness at cellular and subcellular levels. Physiologist, 26 (6 Suppl.), 141 — 142 (1983).
10. Krikorian A. D., O'Connor S. A. Kariological observations. Ann. Bot., 54, 49—63 (1984).
11. Levin H. G., Krikorian A. D. Chromosomes and plant cell division in space environmental conditions and experimental details. Adv. Space Res., 12, 73—82 (1992).
12. Wordragen M. F., Dons H. J. Agrobacterium tumefaciens-mediated transformation of recalcitrant crops. Plant Molecular Biology Reporter, 10 (1), 12—36 (1992).