Plant cell transformation with agrobacterium tumefaciens under clinorotation

1Sarnatzkaya, VV, 1Hladun, HO, 1Padalko, SF
1Institute of Plant Physiology and Genetics, NAS of Ukraine, Kyiv, Ukraine
Kosm. nauka tehnol. 2005, 11 ;(5-6):115-121
Publication Language: Ukrainian
To investigate microgravity (clinorotation) effect on plant cell transformation with Agrobacterium tumefaciens and crown gall tumor formation, the culture of primary explants of potato and Jerusalem artichoke tubers was used. It is found that the efficiency of tumor formation and development in clinorotated explants are considerably reduced. When using the explants isolated from potato tubers clinorotated for 3, 5 and 19 days, drastic reduction of formation and development of crown gall tumors was observed. Conversely, the tumor number and their development were increased when potato tubers were clinorotated for one day. Four-hour clinorotation of explants inhibits chromatin activation (increase in availability of nuclei to acridine orange) and did not induce any appreciable effect on RNA-polymerase I and II. At one-day clinorotation of potato tubers a considerable increase in template accessibility of chromatin and in activity of RNA-polymerase I and II occurred. We discuss the possibility to use short-term clinorotation of plant organs, from which explants for the transformation with A. tumefaciens will be isolated, for the increase in transformation efficiency of recalcitrant plants.
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