Platelets as potential peripheral markers to study functioning of the high-affinity sodium-dependent glutamate transporters in the nerve terminals of the brain
Heading:
| 1Borisova, TA, 2Kasatkina, LA 1Palladin Institute of Biochemistry of National Academy of Sciences of Ukraine, Kyiv, Ukraine 2O.V. Palladin Institute of Biochemistry of the NAS of Ukraine, Kyiv, Ukraine |
| Kosm. nauka tehnol. 2007, 13 ;(2):080-85 |
| https://doi.org/10.15407/knit2007.02.080 |
| Publication Language: Russian |
Abstract: Activity of the high-affinity sodium-dependent glutamate transporters in the brain nerve terminals is demonstrated to alter under artificial gravity conditions. A comparison analyzis is made for L- [14С] glutamate transport in platelets and isolated nerve terminals. The kinetic characteristics of the transporters, [Na+]-dependence and influence of the transporter inhibitor DL-threo-beta-benzyloxyaspartate on the L- [14С] glutamate uptake process are determined. It is shown that glutamate uptake process is very similar for platelets and nerve terminals. Thus it is reasonable to use platelets as a potential peripheral model for glutamate transport. �онкурентного інгібітора DL-трео-бета-гідрокси-аспартату на процес накопичення L- [14С] глутамату тромбоцитами і нервовими закінченнями. Показано, що значення vmaxпроцесу накопичення значно менші у препараті тромбоцитів ніж у нервових закінченнях. Очевидно, це пов'язано з тим, що плазматична мембрана синаптосом містить більшу кількість транспортерів глутамату, ніж мембрана тромбоцитів. У цілому процес накопичення глутамату тромбоцитами і синаптосомами демонстрував значну подібність, і тромбоцити можна використовувати як потенційну периферичну модель транспорту глутамату в ЦНС.
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| Keywords: inhibitor, platelets, transporters |
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