A bacterial consortium attenuates the low-dose gamma-irradiation effect in kalanchoe plantlets

1Burlak, OP, 2Lar, ЕV, 3Rogutskyy, IS, 3Danilchenko, BA, 4Mikheev, OM, 2Zaets, IYe., 5de Vera, J-PP, 6Foing, BH, 2Kozyrovska, NA
1Institute of Molecular Biology & Genetics of NASU, Kyiv, Ukraine
2Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
3Institute of Physics of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
4Institute of Cell Biology & Genetic Engineering of the National Academy of Sciences of Ukraine, Kyiv, Ukraine
5German Aerospace Center (DLR) Berlin, Institute of Planetary Research, FRG, Berlin,Germany
6ESA/ESTEC/SRE-S, postbus 299 NL-2200 AG, Noordwijk, The Netherlands
Kosm. nauka tehnol. 2010, 16 ;(2):75-80
Publication Language: English
The ability of plants to protect themselves against ionizing radiation is limited. One of the ways how to alleviate irradiation consequences in plants is to use plant-associated bacteria for inoculation. Two defined plant growth promoting bacterial strains were used for inoculation Kalanchoe daigremontiana plantlets before acute irradiation with γ-quanta (60 Co). The lethal γ-rays doses were 3.0 kGy for Klebsiella oxytoca IMBG26, and 500 Gy for Paenibacillus sp. IMBG156. The bacteria expressed the increase of the pelX promoter activity after sublethal dose irradiation. The pelX promoter activity that was measured as activity of β-galactosidase of the pelX::lacZ fusion in K. oxytoca (pGalP) was 0,88 mkM/ml·min after exposure to 2.0 kGy, e.a. 80 % of the control (untreated) bacterial activity, although the irradiated bacterial population comprised 1.25 % of control one. Integrated index of plantlets development which was relied on both root number and root length reflected fluctuations in metabolic processes in irradiated plantlets without treatment with bacteria.
          Stabilizing stress-reactions occurred during 10 days after irradiation at different doses (30, 50, 70 Gy), however, index of growth (IG) remained at the level of 30‒60 % to control plantlets. The effect of irradiation on kalanchoe plantlets was relieved by bacteria at doses of 30 and 50 Gy, moreover, IG was observed at levels of 500 and 200 %, respectively. A 30 Gy dose was obviously stimulating for K. daigremontiana plantlets. Intense root elongation, instead of development of new coronal roots, led to fast adaptation to stressful conditions and normalization of metabolic processes in kalanchoe plantlets. However, integrated index showed inhibition of both inoculated and non-inoculated plantlets development after getting a 70 Gy dose.
Keywords: bacteria, gamma-irradiation, inoculation
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