Investigations of the influence of microgravity on the state of human cell lines

1Nosach, LM, 1Povnitsa, OYu., 1Zhovnovata, VL
1Institute of Microbiology and Virology of the National Academy of Sciences of Ukraine, Kiev, Ukraine
Kosm. nauka tehnol. 2004, 10 ;(5-6):226-228
Publication Language: Russian
Clinostating of epithelial (Hela) and limphoblastoid (MT-4) human cells over the period from 96 to 144 hours does not cause considerable changes of morphology and viability of the cells. The proliferative activity of the cells, functional activity of mitochondrions and the capacity of lysosomes to accumulate the fluorochrom are retained.
1. Zelenin A. V. Luminescence Cytochemistry of Nucleic Acids, 136 p. (Nauka, Moscow, 1967) [in Russian].
2. Nosach L. M., Dyachenko N. S., Tarassishin L. O., et al. Determination of the temperature mode, duration of presence of the human adenovirus on orbital space stations and influence of clinorotation on some properties of the virus. Kosm. nauka tehnol., 9 (1), 96—101 (2003) [in Russian].
3. Tairbekov M. G. Gravity cell biology (theory and experiment), 128 p. (Moscow, 1997) [in Russian].
4. Cogoli A., Bechler B., Muller O., Hunzinger E. Effect of microgravity on lymphocite activation. In: Proc. of the Norderney Symposium of scientific Results of the German Spacelab Mission D1, Norderney, Germany, 27-29 August 1986, 366—375 (Germany, 1986).
5. Duke P. J., Montufar-Solis D., Humazaki T., Sato A. Clinorotation of micromass cultures of mouse limb bud cells reduced nodule numbers, bat not size. Abstracts 31-st Scientific Assambly of COSPAR, 14—21 July, 1996, 307 (The University of Birmingham, England, 1996).
6. Kordyum E. Effects of altfered gravity on plant cell processes: results of recent space and clinostatic experiments. Adv. Space. Rev., 14 (8), 77—85 (1994).
7. Kordyum E. Biology of plant cells in microgravity and under clinostating. Internat. Rev. Cyt., 171, 1—78 (1997).
8. Mosmann T. Rapid colorimetric assay for cellular growth and surviral: application to proliferation and cytotoxicity assays. J. Immunol. Methods, 65 (1-2), 55—63 (1983).
9. Pauwels R., Balzarini J., Baba M., et al. Rapid and automated tetrazolium-based colorimetric assay for the detection of anti-HIV compounds. J. of Virological Methods, 20, 309—321 (1988).
10. Rodionova N. V., Oganov V. S. Morpho-functional adaptation in the bone tissue under the space flight conditions. J. Gravit. Physiol., 8 (1), 87—88 (2001).
11. Talas M., Batkai L., Stoger I., et al. Results of space experiment program «Interferon». Acta Microbiol. Hungarica, 30 (1), 53—61 (1983).